The invention discloses a production method of a natural silk cotton quilt and belongs to the technical field of natural silk processing. The method adopts a plant cleaning agent and a plant additive, and comprises the following steps: A, adding the plant cleaning agent into water and adding an old natural silk quilt into the water and boiling; boiling until the water is boiled and keeping for 8min-10min; washing with clean water; B, adding the plant additive into a peeling pool and adding the natural silk quilt in the step A into the peeling pool; peeling layer by layer to form silk sheets and taking out the silk sheets and airing; C, stretching and spreading the silk sheets to form a plurality of layers; paving one layer of cotton wool between each two layers; and D, flattening by a cotton rubbing machine. According to the production method of the natural silk cotton quilt, the problems that an existing natural silk quilt usually becomes yellow and hard after being used for a period of time and needs to be abandoned so that wastes are caused can be solved.