The invention relates to the technical field of antitumor immunity, in particular to an EB (Epstein-Barr) virus specific TCR (T cell receptor) and a recombinant lentiviral vector and application thereof. The EB virus specific TCR comprises an alpha chain and a beta chain. The EB virus specific TCR and the recombinant lentiviral vector and application thereof have the advantages that the EB virus antigen specific TCR is screened, and the EB virus antigen specific TCR is transfected into a T cell by using the lentiviral vector to obtain the T cell with expression aiming at the EB virus specific TCR.