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发明公开
CN106632651A 睫状神经营养因子突变体及其修饰型突变体和应用 有权

睫状神经营养因子突变体及其修饰型突变体和应用
摘要：
本发明涉及一种CNTF突变体及其修饰型突变体和应用。所述CNTF突变体是在天然CNTF的氨基酸序列的基础上发生突变得到的，所述突变包括：第26位的赖氨酸突变为异亮氨酸或亮氨酸、第46位的赖氨酸突变为精氨酸、第160位的赖氨酸突变为谷氨酰胺或蛋氨酸；并且其中所述CNTF突变体保留了天然CNTF第40位的赖氨酸。通过本发明的CNTF突变体能够获得修饰专一性高的修饰型CNTF，从而克服因修饰而带来的修饰产物不均一、结果不稳定等问题，并使重组CNTF蛋白结构稳定，与天然蛋白相比具有更高的生物学活性和可溶性，并且修饰型CNTF与未修饰型CNTF相比具有长效作用。
摘要（英）：

The present invention relates to a CNTF mutant, a modified CNTF mutant, and applications of the CNTF mutant and the modified CNTF mutant. According to the present invention, mutation occurs on the basis of the amino acid sequence of natural CNTF to obtain the CNTF mutant, and comprises the mutation of 26 site lysine into isoleucine or leucine, the mutation of 46 site lysine into arginine, and the mutation of 160 site lysine into glutamine or methionine, and the CNTF mutant retains the 40 site lysine of the natural CNTF; and through the CNTF mutant, the modified CNTF mutant having high modification specificity can be obtained so as to overcome the problems of non-uniform modified product, unstable results and the like caused by the modification, the structure of the recombinant CNTF protein is stable, the recombinant CNTF protein has high biological activity and high solubility compared to the natural protein, and the modified CNTF has a long-acting effect compared to the unmodified CNTF.

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