The invention relates to the technical field of medicines, in particular to application of eupatolide for preparing a liver cancer and colon cancer resisting medicine. The invention has the advantages that the different degree of inhibiting functions of the eupatolide on growth of a human liver cancer cell line BEL-7402 and a human colon cancer cell line HT-29 are proposed for the first time; the growth inhibiting function of the eupatolide on the human liver cancer cell line BEL-7402 and the human colon cancer cell line HT-29 is obviously better than the growth inhibiting function of a positive drug TPT (topotecan hydrochloride); the obvious tumor resisting function of the eupatolide on tumor cells in vitro is realized, and the medical prospect is better.