The invention provides a preparation method of nano-porous glass. The method comprises the following steps: before firing or in a firing process, uniformly mixing a grain-shaped nano-porous material with a glass base material; and then preparing the nano-porous glass according to a conventional process. The method can also be finished through the following steps: after melting glass production raw materials, gradually adding the nano-porous material into melted glass liquid; and preparing glass containing nano pores according to a traditional glass preparation process. The invention further provides glass prepared by the method.