The invention provides a manufacturing method of anti-bacterial undergarments. According to the manufacturing method, by utilizing the sterilization effect of silver particles, a silver-plated shell fabric is used for some parts of the undergarments, so that the undergarments have the anti-bacterial effect. A method for preparing the silver-plated shell fabric provided by the invention comprises the following steps: first, carrying out pretreatment on the shell fabric of the undergarments; then, carrying out dopamine modification on the pretreated shell fabric; and finally, soaking the shell fabric after the dopamine modification in the Tollens' reagent, and carrying out in-situ polymerization silver plating or directly adding with glucose for chemical silver plating. After the silver-plated shell fabric is used for the undergarments, the undergarments have the antibacterial effect. The preparation method provided by the invention is easy to operate and low in cost, the wash resistance is good, and the demands in scientific research and daily life are satisfied.