The invention discloses a water-soluble fluorescent probe for detecting nitric oxide in hepatic cells and application thereof. Compared with the existing fluorescent detecting technology, the fluorescent probe can be quickly positioned in a hepatic cell and can be used for performing in-situ highly-selective real-time detection on nitric oxide in the hepatic cell. Furthermore, the probe has excellent water solubility, optical stability, biocompatibility and higher nitric oxide selectivity, and is not interfered by other common species in organisms, such as active oxygen and active nitrogen. The water-soluble fluorescent probe has huge application prospects in analytical chemistry, life science, medicine and other technical fields. Laser confocal imaging experiments indicate that the probe has relatively good cell permeability and does not have remarkable toxic or side effects on cells and organisms.