The invention discloses a method for detecting the glutathione concentration of a solution. According to the method, a three electrode system is used, cyclic voltammetry is adopted to measure the glutathione concentration of a solution to be measured, the glutathione concentration of the solution can be obtained through a glutathione linear equation; in the three electrode system, the work electrode is subjected to an aminated graphene quantum dot modification treatment, and the work electrode is a glass-carbon electrode. The method has the advantages of simple operation, quick detection, and high sensitivity, and is capable of high-sensitively recognizing glutathione in a mixed sample solution.