The invention provides a flexible conductive MoS2 thermal insulation thin film and a preparation method thereof. The infrared spectrum transmittance of the thin film at the wave band of 1000-1100 cm<1> is 45-56%, the sheet resistance is 50-90 ohm/sq, and the film is flexible and can be bended. The preparation method comprises the following steps: (1) mixing MoS2 powder with a N-methyl pyrrolidone solution, and conducting sealing and water bath ultrasonic treatment to obtain a MoS2-N-methyl pyrrolidone suspension solution; (2) conducting sealing and centrifuging, and removing a longitudinal middle layer solution from the centrifuged solution to obtain the MoS2-N-methyl pyrrolidone suspension solution with a width of 10-15 micrometers; (3) conducting vacuum filtering, peeling off the thin film, and conducting drying to obtain the flexible conductive MoS2 thermal insulation thin film. The thin film is good in stability, flexible, capable of being bended, moderate in sheet resistance value, and capable of absorbing infrared rays radiated by a human body to achieve thermal insulation. The preparation method is simple in technological process, low in cost and suitable for mass production.