The invention relates to a construction method of transgenic rice RCRC02 resisting stem borers and resisting glyphosate, wherein a genetic transformation carrier comprising a glyphosate resistant gene cp4 epsps and a stem borers resistant gene cry1C. Through an agrobacterium tumefaciens mediated genetic transformation method with the glyphosate as a screening agent, T-DNA region of the carrier is successfully single-copied and integrated into genome of rice, so that the transgenic rice plant RCRC02, which resists the stem borers and the glyphosate and has excellent agricultural characters, is cultured. Insertion position of exogenous T-DNA is defined and a specific detection method is designed. The method is suitable of safety evaluation and detection of transgenic rice, and is used for producing various transgenic rice new varieties with the transgenic rice, which is obtained by means of the conversion event, as a donor.