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发明授权
CN106497921B 抗螟虫抗草甘膦转基因水稻KCRC03的构建方法 有权 转让

抗螟虫抗草甘膦转基因水稻KCRC03的构建方法
摘要：
本发明涉及一种抗螟虫抗草甘膦转基因水稻KCRC03的构建方法。本发明构建了包含草甘膦抗性基因cp4epsps和抗螟虫基因cry1C的遗传转化载体，通过农杆菌介导的遗传转化方法、以草甘膦作为筛选剂，成功地将载体的T‑DNA区单拷贝整合到水稻基因组中，并培育出抗螟虫、抗草甘膦、农艺性状优良的转基因水稻植株KCRC03，明确了外源T‑DNA的插入位置并设计了特异性检测方法。本方法适用于转基因水稻的安全评估和检测，以及由该转化事件获得的转基因水稻为供体而得到的各种转基因水稻新品种。
摘要（英）：

The invention relates to a construction method of borer and glyphosate resistant transgenic rice KCRC03. The method comprises the following steps: constructing a genetic transformation vector including glyphosate resistant gene cp4epsps and borer resistant gene cry1C, successfully integrating the T-DNA region of the vector into a rice genome through an Agrobacterium mediated genetic transformation technology by using glyphosate as a screening agent, and cultivating to obtain the transgenic rice KCRC03 with good borer and glyphosate resistance and excellent agronomic properties. The insert position of the exogenous T-DNA is defined, and a specific detection method is designed. The specific detection method is suitable for safety assessment and detection of the transgenic rice. The invention also relates to various new transgenic rice varieties obtained by using the transgenic rice obtained through the above transformation event as a donor.

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