The invention discloses a beauveria bassiana culture method. The beauveria bassiana culturing method comprises the following steps of (1) first-level slant culture: inoculating a beauveria bassiana thallus on a first-level slant culture medium and culturing at the temperature of 24-25 DEG C for 5-6 days; (2) second-level liquid culture: inoculating the strain obtained after the culturing in the step (1) to a seed tank and culturing at the temperature of 24-25 DEG C for 24-28 hours; (3) third-level liquid enlarged culture: inoculating the strain obtained after the culturing in the step (2) to a fermentation tank, and culturing at the temperature of 25-28 DEG C for 28-32 hours; and (4) fourth-level solid culture: inoculating the strain obtained after the enlarged culturing in the step (3) to a solid culture medium and culturing at the temperature of 22-25 DEG C for 5-6 days. By screening and proportioning components of the culture mediums in each stage and cooperating with the culture conditions of temperature and humidity, the culture period is shortened, the quality and the yield of the conidium of beauveria bassiana are improved, and the activity of the conidium is improved.