The invention provides an acidifier preparation method comprising the steps: firstly, adsorbing a short-chain fatty acid and lactic acid with a first carrier to form a core material A; spaying the core material A with a lipid coated material to form a microcapsule acid B; then adsorbing phosphoric acid and/or lactic acid with a second carrier, mixing with a solid organic acid to obtain a powder acid C; then wrapping the microcapsule acid B with the powder acid C to form spherical micro-pellets D; and finally, spraying the surface of the spherical micro-pellets D with the lipid coated material, to obtain the final acidifier product. The method can make the acidifier achieve fixed-point quantitative release, the outermost lipid coated material enters a digestive tract and then can be rapidly dissolved and disintegrated, and then a large amount of phosphoric acid and solid organic acid are released, so as to rapidly reduce the intragastric pH; the lipid coated material outside the inner core and with the short chain fatty acid as the main material is relatively thick, can pass through a stomach, then enters intestinal tracts and slowly releases the short chain fatty acid in the intestinal tracts, so as to inhibit harmful intestinal microorganisms and regulate intestinal microecology balance.