The invention provides a silk screen for silk screen printing and a manufacturing method of the silk screen. Multiple spacer regions and silk thread regions are arranged in the first direction. Silk threads in the first direction are not arranged in the spacer regions. The spacer regions and the silk thread regions are arranged at intervals. In the first direction, the width of the spacer regions is larger than the distance between silk threads in the silk thread regions in the first direction. When a printing screen plate is manufactured, a silk screen provided with the wide spacer regions according to the preset distance is used, according to the preset distance and positions of the spacer regions, a way of a groove thread figure position can be preset in a mask plate, and in the exposure process, photoetching can be conveniently and rapidly implemented only by directly covering the silk screen coated with a photosensitive adhesive with the mask plate and placing the groove thread figure on the mask plate at the position, corresponding to the corresponding spacer region, on the silk screen. Accordingly, the problem that the groove thread position is inconvenient to set when the mask plate is prepared before photoetching is solved, then, the production efficiency of manufacturing the groove line in the screen plate is greatly improved, and meanwhile the material cost spent in the manufacturing process is effectively reduced.