The invention relates to the technical field of drug testing, in particular to a method for detecting a substance inducing anaphylactoid reaction and a kit thereof. The method comprises the following steps: inoculating P815 cells into a culture medium; adding a to-be-detected object and a biological molecule specifically bonded with IP1 and/or cAMP to the culture medium, and detecting concentration of IP1 and/or cAMP; and according to the concentration of IP1 and/or cAMP, determining whether the to-be-detected object is the substance inducing the anaphylactoid reaction or not. The detection method provided by the invention has the advantages that the result is accurate and reliable and is basically consistent with the detection result of the traditional beta-hexosaminidase release rate experiment, but the sensitivity is higher than that of a beta-hexosaminidase release rate detection method; and the detection method provided by the invention is simple and quick, overcomes the defects of the traditional method, can be applied to early stage rapid screening and identification of the substance inducing the anaphylactoid reaction and has extensive value in use.