The invention provides an alternanthera reineckii tissue rapid propagation culture medium. The alternanthera reineckii tissue rapid propagation culture medium includes an initial culture medium, a subculture medium and a rooting culture medium at different stages. The initial culture medium is formed by the steps of adjusting the concentration of ferrous sulfate to 15-18 mg/L on the basis of an SH culture medium, then replacing potassium iodide with potassium iodate with a concentration of 2.0-2.2 mg/L and in addition, adding riboflavin, ZT and ascorbic acid; the subculture medium is formed by the steps of adjusting the concentration of ferrous sulfate to 22-25 mg/L on the basis of one SH culture medium, then replacing potassium iodide with potassium iodate with a concentration of 2.5-2.8 mg/L and in addition, adding ginkgo leaf polysaccharide, TDZ and amylopectin; the rooting culture medium is formed by the steps of adjusting the concentration of ferrous sulfate to 25-30 mg/L on the basis of one SH culture medium, then replacing potassium iodide with potassium iodate with a concentration of 3.0-3.2 mg/L and in addition, adding forchlorfenuron, IBA and activated carbon. The alternanthera reineckii tissue rapid propagation culture medium is designed based on the growing characteristics of glossostigma elatinoides, and can meet the nutritional requirements at various stages of glossostigma elatinoides tissue culture.