The invention discloses a method for directly inducing a tobacco leaf regenerated plant by using spermine. The method comprises the following steps: taking a tobacco leaf explant; inoculating into an MS solid culture medium with the spermine; adjusting a pH value to be 5.8 to 6.0; culturing for 30 days to obtain the tobacco leaf regenerated plant. According to the method disclosed by the invention, the preparation of the culture medium can be simplified; the method has the advantages of simple operation, high inducing speed of the regenerated plant, high yield and good quality.