A fixture for testing low-temperature peel strength of adhesive comprises a cuboid support and support rollers mounted on the support, wherein the cuboid support is provided with a vertical screw at the center of the lower surface; each support roller is composed of a pair of support sheets respectively fixed to the front and rear sides of the support, support roll shafts mounted between the support sheets and roll barrels sleeving the roll shaft, and the roller barrels include an upper horizontal roll shaft and a lower horizontal roll shaft; the support rollers include fixed support rollers and sliding support rollers; the fixed support rollers are mounted at the center line of the vertical screw and on the front and rear sides of the cuboid support on the left of the vertical screw; the front and rear sides of the support on the right of the vertical screw are provided with horizontal slide groove guide rails; convex sliders are embedded into slide grooves; the sliding support rollers are fixedly mounted on the front and rear sides of the cuboid support on the right of the vertical screw through the convex sliders; a sample is placed in a gap between the rollers vertically fixed parallelly. The fixture has the advantages that rolling guidance is accurate, peel angle is adjustable, mounting and testing are facilitated, and the fixture is suitable for testing the low-temperature peel strength of the adhesive.