The invention relates to a lifting hook for lifting an artificial fish reef and an application method thereof. The lifting hook comprises a main steel wire rope, branch steel wire ropes and two lifting hooks of the same structure, wherein the lifting head part at the lower end of the opening of the lifting hook is fixedly provided with a traction steel chain with a guide lead block respectively. The application method of the lifting hook comprises the following steps: (1) keeping the openings of the two lifting hooks inward to hook hanging rings at both sides of the top part of an artificial fish reef; (2) threading the traction steel chain from the inner side of the hanging ring to the outer side by operating personnel, so that the guide lead block naturally drops along the outer side wall of the artificial fish reef; (3) lifting the artificial fish reef, placing at the bottom of sea, and further lowering the main steel wire rope to realize unhooking of the lifting hooks and the hanging rings; (4) enabling the main steel wire rope to make a horizontal movement, so that the lifting hooks and the hanging rings are twisted away from each other in the vertical direction; and (5) raising the main steel wire rope to finish lifting. According to the invention, the lifting hook is reasonable in design, and the structure is easy to implement; and the method can be quickly and conveniently operated, and is especially applicable to lifting and unhooking in a turbulence disturbing water body.