The invention discloses a living kidney tissue puncturing sampling needle. The needle comprises a hollow puncturing tube, wherein a sampling hole is formed in the tube wall at the front end of the hollow puncturing tube, and a sample storing tube is installed in the tube in a sleeving manner; a sample storing groove is formed in the tube wall at the front end of the sample storing tube, and the edge of the groove is subjected to edge opening; the sampling hole and the sample storing groove are formed in the same axial position; the end part of the hollow puncturing tube is of a solid sharp tip structure. By rotating a rotary cut tube in the middle, a sample falling into the sample storing groove is separated from a boxy, and the wound area is small. The end part of the hollow puncturing tube is of the solid sharp tip structure, and histocyte can be prevented from entering a crack between the sample storing tube and the hollow puncturing tube when Lesion organs are punched, so that puncturing is facilitated. The needle can be used for well solving the problems of complex operation and large internal wound area of the existing kidney tissue puncturing sampling needle.