The invention discloses a fastener fatigue test loading frame capable of adapting to various loading angles. The fastener fatigue test loading frame comprises a bearing cross beam, two sliders, two support lug welding parts, two limit blocks and two fixing shafts, wherein the bearing cross beam is horizontally arranged, and rectangular square holes are bilaterally symmetrically formed in the bearing cross beam; the sliders are assembled in the rectangular square holes; the two fixing shafts penetrate through the two sliders respectively; top ends of the two support lug welding parts are connected with the bearing cross beam through the fixing shafts; tail parts of the fixing shafts penetrate out of the support lug welding parts and are equipped with retaining shaft sleeves; contact angles are formed at the bottom ends of the support lug welding parts; the two limit blocks are symmetrically arranged on the bottom surface of the bearing cross beam; side surfaces of the limit blocks are manufactured to form corresponding oblique angles; long bolts are arranged on the bottom surfaces of two ends of the bearing cross beam; nuts are arranged at bottoms of the long bolts and used for liming opening angles of the support lug welding parts. The fastener fatigue test loading frame has the beneficial effects of being convenient to adjust and facilitating improvement of the test efficiency.