The invention discloses a method for synthesizing liquid fuel containing dioxolane with biomass material one-pot reaction. The method includes: adding biomass materials into a reactor; adding a solvent and extraction agent cyclohexane in the reactor, and meanwhile adding a catalyzer; sealing the reactor and emptying air in the reactor; then filling biomass gas and raising the temperature to 80-300DEG C with reaction time being 1-72 hours; after completion of reaction, cooling to indoor temperature, wherein the extraction agent cyclohexane is layered with the solvent, and the layered extraction agent cyclohexane is taken out to be distilled to have cyclohexane removed to obtain the product which can serve as the liquid fuel for direct use.