The invention provides a negative dielectrophoresis hydrocyclone and relates to a hydrocyclone. The negative dielectrophoresis hydrocyclone comprises a lower tank body, a middle tank body component, an upper tank body and an overflow pipe component. In the dielectrophoresis process of the negative dielectrophoresis hydrocyclone, negative dielectric property particles absorbed to the radial inner side of an electrode 2 and positive dielectric property particles absorbed to the radial outer side of an electrode 1 can be rapidly discharged out of the negative dielectrophoresis hydrocyclone by using the kinetic energy of inlet liquid rotational flow. Through the mutual cooperation of the spiral structures of the electrode 2 and the electrode 1 and the inlet liquid rotational flow, the dwell time of a liquid medium in an electrophoresis cavity is prolonged, so that the separating efficiency of the negative dielectrophoresis hydrocyclone is increased.