The invention discloses a high quality reconstruction method for loss spots in the STED super-resolution microscopic technology. An unloaded sample beam goes through uniform phase spatial light modulation and then is focused by an objective lens, and ideal loss spots are obtained in a focal plane; a loaded sample beam goes through uniform phase spatial light modulation and then is focused by the objective lens, and distortion loss spots are obtained in a focal plane at the internal part of a sample; pixel points of a spatial light modulator are partitioned, different phase values are loaded to partitions, and a series of loss spots to be corrected are acquired, then cross-correlation calculation and processing are carried out via the loss spots to be corrected and the ideal loss spots, and the phase loading optimum value of each partition is obtained; after several times of iterative processing in each partition, high quality reconstruction of the loss spots at the internal of the sample is completed. The high quality reconstruction method is advantageous in that, loss hollow spots can be reconstructed, complete and good loss spots can be obtained at large depth, application range of stimulated radiation quenching microscopic technology is expanded, system imaging depth is improved, system resolution and signal-to-noise ratio are improved, and imaging quality is improved.