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发明公开
CN106282101A 一种促进人羊膜间充质干细胞向软骨细胞分化的方法及应用 无效 - 驳回

一种促进人羊膜间充质干细胞向软骨细胞分化的方法及应用
摘要：
本发明涉及一种促进人羊膜间充质干细胞向软骨细胞分化的方法，该方法为在人羊膜间充质干细胞中添加诱导组合物，所述诱导组合物包括0.01～2g/L透明质酸和0～100mg/L抗坏血酸。本发明与常规诱导方法相比，可显著提高人羊膜间充质干细胞向软骨细胞分化，糖胺聚糖和II型胶原蛋白表达呈强阳性，Col2α1、ACAN和Sox9等软骨形成相关基因和蛋白的表达亦明显上调，能明显提高人羊膜间充质干细胞向软骨细胞分化的效率。本发明中诱导组合物可用于人间充质干细胞向软骨细胞分化中以及在软骨损伤疾病中。
摘要（英）：

The invention relates to a method for promoting differentiation of human amniotic mesenchymal stem cells into chondrocytes. The method comprises the step of adding an induction composition into the human amniotic mesenchymal stem cells, wherein the induction composition comprises 0.01-2g/L of hyaluronic acid and 0-100mg/L of ascorbic acid. Compared with the conventional induction method, the method disclosed by the invention has the advantages that the effect of promoting differentiation of human amniotic mesenchymal stem cells into chondrocytes can be obviously improved, glycosaminoglycan and type II collagen expression is strongly positive, expression of chondrogenesis related genes and proteins such as Col2alpha1, ACAN, Sox9 and the like is obviously improved, and the efficiency of differentiating the human amniotic mesenchymal stem cells into chondrocytes can be obviously improved. The induction composition disclosed by the invention can be used for differentiation of human amniotic mesenchymal stem cells into chondrocytes and cartilage injury diseases.

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