The invention discloses a Nocardia farcinica bacteria strain and application thereof and belongs to the field of pesticide. The Nocardia farcinica bacteria was conserved in Common Microorganism Center of China Microorganism Bacteria Conservation Management Committee with the conservation number of CGMCC NO.12344 in 11th, Apr, 2016. The address of the conservation unit is: No.3, No.1 yard, Beichen Rd. (West), Chaoyang District, Beijing City, Microorganism Research Institute of Chinese Academy of Sciences. The Nocardia farcinica bacteria strain is applied to prepare a biological medicine to poison a southern root-knot nematode J2. The bacteria's advantage is in that, The Nocardia farcinica bacteria poisons the southern root-knot nematode J2 fast, has high control effect, has no pollution for agricultural product, human, animal and environment; and can be used for preparing biological medicine.