The invention discloses a die for producing a computer display screen base and a guide structure thereof. The die comprises a guide column arranged on a movable die and a guide sleeve arranged on a fixed die and matched with the guide column, the guide column comprises a fixed column fixed to the movable die and at least one movable column in sliding fit with the fixed column; a buffering portion supportive of being squeezed by the movable column and axially deforming and being compressed is arranged in the guide sleeve, in this way, sliding distance at the end of the guide column is dispersed on the whole guide column, burden at the end of the guide column is reduced, and local wear is reduced, so that matching smoothness of the guide column and the guide sleeve is improved; a one-way air incoming structure and a one-way air outgoing structure are arranged between a sliding block on a penetrating column and a sliding cavity, so that speed at which the guide column is inserted into the guide sleeve can be lowered in the process of die assembly, heat yield and wear between the guide column and the guide sleeve can be lowered, and practical effect of the guide structure is further improved while service life of the same is further prolonged, so that quality of the display screen base produced by injection molding is improved.