The invention belongs to the field of analysis detection, and more specifically relates to a magnetic nanometer microsphere adsorption method for nicotine in tobacco leaves. The tobacco leaves and the magnetic nanometer microspheres are placed in the same enclosed container for 12-48 hours. The magnetic nanometer microsphere adsorption method for nicotine in tobacco leaves is advantageous in that (1) the magnetic microspheres have ferromagnetism, and applied magnetic field can be used for conveniently separating the microspheres from a mixture; (2) mesoporous SiO2 enhances adsorption area of the magnetic material, and can be effectively combined with a carbon chain in N-propyl ethene diamine; (3) N-propyl ethene diamine can enhance monodispersion of magnetic microspheres in a solution/solvent, and can strengthen adsorption for polar organic compounds; (4) Zr-MOF at the external layer and heterocycles in nicotine can form pi-pi and p-pi combinations, in order to realize good adsorption effects.