The invention provides an artificial vertebra by anterior pedicle screw fixation and a positioning assembly tool. The artificial vertebra is characterized in that a pedicle screw fixing hole is formed in the artificial vertebra, and the screw is accurately guided to fix the artificial vertebra on two bilateral vertebral pedicles to realize firm self-stabilization. According to the artificial vertebra, a steel plate can be integrated into the artificial vertebra according to requirements, upper and lower ends of the steel plate exceed the length of the artificial vertebra, or only the upper end of the steel plate is longer than the artificial vertebra; the long end of the steel plate is provided with a screw hole coaxial with the vertebral pedicle and a screw hole oriented to the pedicle; a drill positioning guide plate and an osteotomy guide plate are simultaneously provided to ensure that an anterior pedicle screw can be safely and accurately implanted; and the artificial vertebra as well as the drill, osteotomy guide plate and other assembly tools thereof are designed and printed by utilizing a 3D printing technology based on medical imaging data. By adopting the artificial vertebra, the fixing strength of anterior spine stability can be maximized safely and quickly.