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发明授权
CN106191052B 启动子17M11D及其应用 有权 转让

启动子17M11D及其应用
摘要：
本发明公开了启动子17M11D及其应用。本发明提供的启动子，命名为17M11D，为序列表的序列1自5’末端第7至94核苷酸所示的DNA分子。本发明还保护一种DNA分子(融合基因)，自上游至下游依次包括序列表的序列1自5’末端第7至94核苷酸所示的DNA分子和报告基因。本发明还保护含有所述融合DNA的重组质粒。本发明还保护将所述重组质粒导入宿主菌得到的重组菌。本发明还保护所述重组菌在检测2,4,6‑三硝基甲苯和/或2,6‑二硝基甲苯和/或甲苯和/或2,4‑二硝基甲苯和/或1,4‑二硝基苯中的应用。本发明提供的生物传感器不仅可应用于战时及战后地雷等爆炸物位置的检测鉴定，还为土壤及水环境中的TNT检测提供新的方法。
摘要（英）：

The invention discloses a promoter 17M11D and applications of the promoter 17M11D. The promoter provided by the invention is named as 17M11D, and is DNA molecules shown by the nucleotide from the 7th site to the 94th site of the 5' terminal in the sequence 1 of the sequence table. The invention also protects the DNA molecules (fusion genes), sequentially comprising the DNA molecules shown by the nucleotide from the 7th site to the 94th site of the 5' terminal in the sequence 1 of the sequence table and reporter genes from the upstream to the downstream. The invention also protects recombinant plasmids containing the fusion DNA. The invention further protects recombinant bacteria obtained by importing the recombinant plasmids into host bacteria. The invention also protects the applications of the recombinant bacteria in detecting 2,4,6-trinitrotoluene and/or 2,6-dinitrotoluene and/or methylbenzene and/or 2,4-dinitrotoluene and/or 1,4-dinitrobenzene. A biosensor provided by the invention can be used for detecting and identifying the position of the explosive, such as the mine during the wartime and after the war, and moreover, a novel method is provided for detecting TNT in the soil and the water environment.

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