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发明授权
CN106191051B 启动子7M4D及其应用 有权 转让

启动子7M4D及其应用
摘要：
本发明公开了启动子7M4D及其应用。本发明提供的启动子，命名为7M4D，为序列表的序列1自5’末端第7至93位核苷酸所示的DNA分子。本发明还保护一种DNA分子(融合基因)，自上游至下游依次包括序列表的序列1自5’末端第7至93位核苷酸所示的DNA分子和报告基因。本发明还保护含有所述融合DNA的重组质粒。本发明还保护将所述重组质粒导入宿主菌得到的重组菌。本发明还保护所述重组菌在检测2,4,6‑三硝基甲苯和/或2,6‑二硝基甲苯和/或甲苯和/或2,4‑二硝基甲苯和/或1,4‑二硝基苯中的应用。本发明提供的生物传感器不仅可应用于战时及战后地雷等爆炸物位置的检测鉴定，还为土壤及水环境中的TNT检测提供新的方法。
摘要（英）：

The invention discloses a promoter7M4D and an application thereof. The provided promoter is named as 7M4D, and is the DNA molecule shown as 7th to 93rd nucleotide from a 5' tail end of a sequence 1 in a sequence table. The invention also provides the DNA molecule (fusion gene) which comprises the DNA molecule shown as 7th to 93rd nucleotide from a 5' tail end of the sequence 1 in the sequence table and a report gene from upstream to downstream in order. The invention also provides the recombinant plasmid containing the fusion DNA. The invention also protects recombinant bacteria obtained by introducing the recombinant plasmid to a host bacterium. The invention also protects the recombinant bacteria in an application for detecting 2,4,6-trinitrotoluene and/or 2,6-dinitrotoluene and/or toluene and/or 2,4-dinitrotoluene and/or 1,4-dinitro benzene. A biosensor provided in the invention can be used for detection and identification of position of the explosives such as land mine in wartime and after war, and also provides a novel method for detecting TNT in soil and water environment.

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