The invention discloses a method for preparing a high-purity standard ginkgo leaf extract. The preparation method comprises the steps of weighing raw materials, smashing and sieving the raw materials, and placing the raw materials into an extracting reflux tank; performing reflux extraction; recycling alcohol, performing vacuum concentration to obtain extract with a specific gravity of 1.0-1.5; using pure water to fully stir the extract, performing high-speed centrifugation to remove a precipitate, and obtaining supernatant; causing the supernatant to pass macroporous resin, performing gradient elution, collecting 40-60% of alcohol eluent, and then performing acidification to obtain acidizing fluid; precipitating the acidizing fluid at 0-5 DEG C and staying overnight, and collecting the precipitate; using pure water to fully wash the precipitate, and then drying the precipitate under vacuum conditions to obtain the standard ginkgo leaf extract. A device adopted for the method is simple, and the method is simple and feasible in process and facilitates industrial production; a used solvent is non-toxic and free of residue and does not cause environmental pollution; the injurant removal rate of the ginkgo leaf extract is high, and the produced standard extract conforms to the American FDA requirements.