The invention discloses a method for elimination and inhibition of hydrogen sulfide in oil well production. The method comprises a measure for processing a water well tube pillar and a water injection near-well zone, as well as a measure for processing an oil well tube pillar and a near-well zone of oil well tube pillar. The method disclosed by the invention has the advantages that generation of the hydrogen sulfide during the oil well production can be eliminated and inhibited efficiently and safely; the method has good effects and a long-term measure validity period especially for the inhibition of hydrogen sulfide which is generated from bacteria breeding; the maximum decrease rate of the hydrogen sulfide concentration reaches 99.68%; the method disclosed by the invention can obviously reduce the hydrogen sulfide concentration at different well temperatures; and a green and environment-friendly bactericide can be selected during biologic inhibitor selection of the method disclosed by the invention, so that fewer influences are brought to environments. The method is an economic, practical and environment-friendly technological measure for controlling the hydrogen sulfide generation.