The invention discloses a dialysis device and a micro-universe experimental device including the dialysis device. The dialysis device comprises a dialysis bag and bottom-off centrifugal tubes, wherein each bottom-off centrifugal tube comprises a tube cap and a tube thread; the dialysis bag is distended in a cylinder body, and both ends of the cylindrical dialysis bag are sealed respectively through the tube caps and the tube threads of the bottom-off centrifugal tubes. The micro-universe experimental device includes the dialysis device and a culture container, and the dialysis device is placed in the culture container. The device is used to study the indirect effects of interspecific relationships in aquatic ecosystems and can be applied to scientific experiments and special cultivation of plankton. The dialysis device and the micro-universe experimental device are conducive to conducting relevant researches with scientific value and social benefits.