A puncture device sealing membrane comprising a groove structure. A sealing film (130) comprises a proximal opening (132), a distal opening (133), and a sealing wall (135) extending from the distal opening (133) to the proximal opening (132). The sealing wall (135) comprises a proximal end surface and a distal end surface. The distal opening (133) is formed by a sealing lip (134), and is used for receiving a device inserted therein and forming a seal. The sealing lip (134) comprises a central axis (158) and a transverse plane (159) generally perpendicular to the axis (158). In the vicinity of the sealing lip (134), the sealing wall (135) comprises a main rotary wall (138) and a plurality of grooves (140). Each of the grooves (140) comprises two side walls (142), and the side walls (142) are areas which are defined on both sides, which extend laterally and outwardly from the sealing lip (134) and which have a gradually increasing width. The grooves (140) feature functions such as increasing a circumferential length, reducing a wrapping area, improving lubrication reliability, and increasing axial tensile rigidity, thereby greatly reducing frictional resistance and stick-slip.