知搜-全球专利检索

  1. 登录
  2. 注册
发明公开
CN106133141A RNA引导的基因驱动 有权

RNA引导的基因驱动
摘要：
公开了RNA引导的Cas9基因驱动及其使用方法。本发明的实施方式是基于使用RNA引导的DNA结合蛋白与引导RNA在靶DNA位点处共定位并发挥基因驱动的作用。本发明范围内包括的DNA结合蛋白包括由RNA引导的那些，在本文中称为引导RNA。DNA结合包括天然产生的具有核酸酶活性的DNA结合蛋白，如Cas9蛋白。根据一个方面，本发明的酶，如Cas9展开DNA双链体并且检索与cdRNA匹配的序列以切割。
摘要（英）：

RNA guided Cas9 gene drives and method for their use are disclosed. Embodiments of the present disclosure are based on the use of RNA guided DNA binding proteins to co-localize with guide RNA at a target DNA site and act as gene drives. DNA binding proteins included within the scope of the present disclosure include those which may be guided by RNA, referred to herein as guide RNA. DNA binding include naturally occurring DNA binding proteins having nuclease activity, such as Cas9 proteins. According to one aspect, the enzyme of the present disclosure, such as Cas9 unwinds the DNA duplex and searches for sequences matching the crRNA to cleave.

公开/授权文献
0/0