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发明公开
CN106086025A 一种具有启动子功能的DNA片段及其应用 失效 - 权利终止

一种具有启动子功能的DNA片段及其应用
摘要：
本发明公开了一种具有启动子功能的DNA片段及其应用。该DNA片段为如下任一序列：(a)如SEQ ID NO.1所示的核苷酸序列或者其互补序列；(b)对如SEQ ID NO.1所示的核苷酸序列进行一个或多个核苷酸取代、缺失或添加所获得的，具有与如SEQ ID NO.1所示的核苷酸序列相同的作为启动子功能的核苷酸序列或者其互补序列；(c)对如SEQ ID NO.1所示的核苷酸序列添加一个或多个核糖体结合位点的序列。该DNA片段具有启动子的功能，有很强的特异表达活性，在不需要添加诱导物的条件下即能实现外源基因的高表达，特别是为枯草芽孢杆菌表达外源基因提供了有效的元件。
摘要（英）：

The invention discloses a DNA fragment with a promoter function and a use thereof. The DNA fragment has a sequence selected from (a), a nucleotide sequence shown in the formula of SEQ ID NO. 1 or its complementary sequence, (b), a nucleotide sequence which is derived from the nucleotide sequence shown in the formula of SEQ ID NO. 1 through replacement, deletion or addition of one or multiple nucleotides and has a promoter function the same to that of the nucleotide sequence shown in the formula of SEQ ID NO. 1, or its complementary sequence, and (c), a sequence derived from the nucleotide sequence shown in the formula of SEQ ID NO. 1 through addition of one or more ribosome binding sites. The DNA fragment has a promoter function and strong specific expression activity, realizes high exogenous gene expression without an inducer and provides an effective element for bacillus subtilis expression of an exogenous gene.

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