The invention belongs to the technical field of microorganisms, and discloses an Aspergillus niger strain. The strain is identified as Aspergillus niger Z-9, and has the preservation number CGMCC No.11130 in the Common Microorganism Center of the CCCCM (China Committee for Culture Collection of Microorganisms). The invention further discloses application of the Aspergillus niger. By fermentation, the Aspergillus niger strain can generate cellulase, pectinase and xylanase; the Aspergillus niger strain can be used for preparing a cellulase, xylanase or pectinase preparation, and also can be used for preparing a composite enzyme preparation at least containing the cellulase, the xylanase or/and the pectinase, and an application range of the Aspergillus niger strain in a feed enzyme preparation is expanded; the Aspergillus niger strain can be used for a biological fermentation feed, pomace substances can be effectively modified into a biological protein feed, the added value of pomace is promoted, excellent materials are provided for the feed application of the pomace substances, and the prepared biological fermentation feed has an excellent application effect of feeding animals, e.g., cows, sheep, piglets and the like.