The invention provides an automatic power supply cable reel positioning device and relates to the field of storage devices. The automatic power supply cable reel positioning device comprises a chassis, wherein a second slide rail is arranged on one side of the chassis, a push trolley is arranged on the second slide rail, a cable reel is placed on the push trolley, a cable shaft is arranged on the cable reel, multiple second guide wheels are arranged on the lower side of the push trolley and are in contact with the second slide rail, two installing grooves are formed in the push trolley, clamping teeth are arranged on the side face of the push trolley, an installing strip is arranged on the chassis, a moving strip is arranged on the installing strip, cam clamping mechanisms are arranged on the push trolley, and the cable reel is limited on the push trolley by the four cam clamping mechanisms. The problem of potential safety hazard produced by accidental cable reel rolling occurred due to unfirm fixing or supporting object looseness during lead reel storage can be solved, the problems of non-standard placement and irregular appearance are solved, automatic control is adopted, and casualties caused by manual cable reel movement are avoided.