The invention discloses a making method of yak skimmed milk mucormycosis soft cheese. The making method comprises the steps that yak milk is filtered, antibiotic detection and pH value detection are conducted, and centrifugal degreasing is conducted; yak skimmed milk is sterilized and cooled down, and starter culture and mucormycosis are added for fermentation; 0.02% of CaCl2 is added, and after 10 minutes, 0.003% of rennin is added; curds are cut and stirred, and small blocks of the curds of which most whey is separated out are put into a mold for whey draining; after the mold is turned upside down, whey draining is conducted continuously, and the curds are cut into blocks and soaked in a saturated salt solution; upside down turning is conducted once every day under the conditions of the temperature of 15 DEG C and the relative humidity of 90%, and maturation in the late stage takes seven days. According to the making method of the yak skimmed milk mucormycosis soft cheese, the original nutritional ingredients of the yak milk are preserved, by means of the addition and fermentation technology of lactic acid bacteria, the digestibility of a human body is increased, the flavor substance of the product is enhanced, the mouthfeel of the product is improved, and the additional value of the product is improved.