The invention discloses a rhodiola extract niosome and a preparation method thereof. By modified film dispersion-high pressure homogenization, a rhodiola extract niosome suspension is prepared; and by a freeze drying method, a niosome freeze-dried powder is prepared. Raw materials are composed of the following ingredients (by weight): 1-10 parts of a rhodiola extract, 4-60 parts of a nonionic surfactant, 3-50 parts of cholesterol and 5-20 parts of a freeze-drying protective additive. The rhodiola extract niosome preparation has abilities of prolonging drug duration in vivo, avoiding phagocytosis by reticuloendothelial system of liver tissue and assisting active components of rhodiola to pass through blood brain barrier. The prepared rhodiola extract niosome has good stability. The technology is simple and is suitable for industrial production.