知搜-全球专利检索

  1. 登录
  2. 注册
发明授权
CN106030307B 用于确定基因组完整性和/或通过确定性限制位点全基因组扩增获得的DNA序列的文库质量的方法和试剂盒 有权

用于确定基因组完整性和/或通过确定性限制位点全基因组扩增获得的DNA序列的文库质量的方法和试剂盒
摘要：
本发明涉及用于确定样品基因组的完整性和/或由样品的基因组的确定性限制位点全基因组扩增获得的DNA序列的文库的质量的方法，包括以下步骤：(a)提供DNA序列的文库：(b)使用至少一个第一引物对、一个第二引物对和一个第三引物对通过PCR扩增DNA序列的文库，引物对各自杂交至具有1000bp至5000bp长度并对应分别位于第一、第二和第三染色体臂上的基因组的序列的文库的DNA序列，扩增的步骤产生各自为50bp至1000bp并各自具有与其他不同的尺寸的第一、第二和第三PCR产物；(c)检测第一、第二和第三PCR产物；(d)将第一、第二和第三PCR产物的存在与样品的基因组的完整性和/或DNA序列的文库的质量相关联。
摘要（英）：

The present invention relates to a method for determining the integrity of the genome of a sample and/or the quality of a library of DNA sequences obtained by deterministic restriction site whole genome amplification (DRS-WGA) of the genome of the sample comprising the steps of: (a) providing the library of DNA sequences; (b) amplifying the library of DNA sequences by PCR using at least one first primer pair which hybridises to a DNA sequence of the library having a length from 1000 bp to 5000 bp and corresponding to a sequence of the genome located on a first chromosome arm, the step of amplifying giving rise to a first PCR product from 50 to 1000 bp; (c) detecting the first PCR product; (d) correlating the presence of the first PCR product with the integrity of the genome and/or the quality of the library of DNA sequences. The present invention further relates to a related kit and uses thereof.

公开/授权文献
0/0