The invention belongs to the technical field of plant tissue culture and in particular relates to a tissue culture rapid propagation method of Xianwang jujubes. The method comprises the steps of explant establishment, initiation culture, subculture, rooting culture, hardening and transplanting. The method has the beneficial effects that researches are conducted aiming at the variety of Xianwang jujubes and the most appropriate culture medium composition is adopted at different stages, so the propagation coefficient is high and the rooting rate is high; the method is simple and convenient to operate, is low in cost and can achieve the effect of effectively and conveniently obtaining plenty of Xianwang jujube tissue culture seedlings in a short time; the obtained tissue culture seedlings have high survival rates and can maintain good genetic characters of superior trees; plenty of uniform regeneration plants with stable genetic characters can be obtained by adopting the tissue culture rapid propagation method of Xianwang jujubes, have stable test results and good repeatability, can be applied to large-scale commercial seedling raising and are suitable for being planted in large-area regions in the northern mountain regions in Guangxi, especially in the mountain regions with karst landforms.