The invention discloses an eosin staining solution. The eosin staining solution is prepared from the following components in parts by mass: 12 parts of water-soluble eosin, 1200 parts of water, 0.4 part of sodium chloride, 1.3 parts of a 1mol/L citric acid water solution, 20 parts of absolute ethyl alcohol, 0.01 part of nano silver, 19 parts of glycerol, 2 parts of a boric acid solution with the concentration of 5mmol/L, 2 parts of stearic acid, 0.3 part of cetyl alcohol, 1 part of isopropanolamine, 2 parts of Tween 20, 0.8 part of allantoin, 1 part of ethylparaben, 6 parts of acrylic resin, 0.8 part of tocopheryl acetate, 0.001 part of sodium dodecylbenzene sulfonate and 0.001 part of fatty glyceride. The eosin staining solution meets the requirement of environment protection of pathological reagents and the staining effect is better than that of a traditional eosin staining solution.