The invention relates to an automatic closed loop joint debugging method for telecontrol equipment and a regulation and control main station of an intelligent substation. According to the automatic closed loop joint debugging method, The correctly-debugged SCD configuration files from the monitoring background of the substation and the EXCEL model files issued by the regulation and control main station are taken as the standard basis data source; data source simulation generation software based on the existing IEC61850 protocol is adopted for simulating the operations of sending the telemetry and telecontrol data of the substation to the telecontrol equipment, obtaining messages which are sent to the regulation and control center from the telecontrol equipment, performing analyzing, comparing and the like on the messages, and realizing telemetry and telecontrol automatic closed loop joint debugging; and meanwhile, the software automatically simulates the operations of sending down the telecontrol instruction from the regulation and control center to the telecontrol equipment, obtaining the telecontrol data which is sent down to the substation from the telecontrol equipment, performing analyzing, comparing and the like on the messages, and realizing telecontrol automatic closed loop joint debugging. According to the automatic closed loop joint debugging method, the purpose of joint debugging of the telecontrol equipment and the regulation and control main station can be fulfilled without requiring power failure of primary and secondary equipment; the accuracy can achieve 100%; the time saving rate can reach greater than 95%; and therefore, the working efficiency is greatly improved, and the reliable and safe operation of the power grid can be ensured.