The invention discloses a chicken egg-yolk antibody capable of resisting influenza virus. The chicken egg-yolk antibody is used for specifically resisting tetravalence influenza virus. The influenza virus includes influenza A virus subtype H1N1, influenza A virus subtype H3N2, influenza B virus subtype Victoria series and influenza A virus subtype Yamagata series. The preparation method of the chicken egg-yolk antibody comprises the following steps of 1, preparing a tetravalence influenza virus antigen; 2, performing immunity on hens; 3, collecting immunized eggs laid by the immunized hens; 4, preparing a mixed antibody stock solution from the immunized eggs; 5, detecting titer of the antibody stock solution, and a stabilizer is added cooperatively, so that the chicken egg-yolk antibody capable of resisting tetravalence influenza virus is obtained. The chicken egg-yolk antibody can be combined with the tetravalence influenza virus specificity pathogen for a short period of time, the tetravalence influenza virus can be effectively killed for a long period of time, the safety performance is good, no toxic or side effect is generated, and the chicken egg-yolk antibody can be effectively used for preventing pathogen corresponding to human pharyngeal and nasopharynx mucosas.