The invention discloses a fermentation production method of bacterial cellulose. The method is a combined method for dynamically cultivating thalli and statically fermenting bacterial cellulose and is applied to two closed and multi-layered bacterial cellulose culture devices. The inoculated culture medium is cultured for 12-24 h through a dynamic method, and a fermentation mother solution with the certain amount of thalli is obtained. Then, a peristaltic pump and other devices are used for injecting the fermentation mother solution into the two bacteria cellulose stationary culture device from top to bottom through a pipeline on the aseptic condition, and finally gel bacterial cellulose is obtained. The method can effectively control the fermentation conditions and the thickness of a bacterial cellulose membrane, meanwhile, the contamination probability is lowered, the labor force is saved, finally, the fermentation production efficiency is improved, and the production cost is lowered.