The invention discloses a self-adhesion cold sealing adhesive containing no protein component as well as a preparation method and an application of the self-adhesion cold sealing adhesive, and belongs to the technical field of medicine packaging. The self-adhesion cold sealing adhesive containing no protein component is prepared from main raw materials including synthetic latex, filler and auxiliaries. The preparation method of the self-adhesion cold sealing adhesive comprises the following steps: the raw materials are weighed in proportion, mixed and stirred. The self-adhesion cold sealing adhesive can be taken as an adhesive for internal wrapping paper of an adhesive bandage, and specifically, the cold sealing adhesive is directly applied to the paper and dried. The cold sealing adhesive can effectively solve the problem of protein allergy caused by the cold sealing adhesive prepared from natural latex, thereby facilitating expansion of Chinese medicine products such as adhesive bandages and the like in European and American markets.