The invention relates to the field of rubber preparation, in particular to rubber composition containing a silane coupling agent and carbon nanotubes and a preparation method of the rubber composition. The rubber composition is prepared from substances as follows: rubber, the silane coupling agent, 0.1-5 parts by weight of the carbon nanotubes, white carbon black and an aid, wherein the aid is prepared from a softener, an anti-ageing agent, sulfur and a vulcanization accelerator. The preparation method comprises steps as follows: the rubber, the silane coupling agent, the carbon nanotubes and the white carbon black are added to water to be mixed, and a suspending liquid is prepared; a flocculant is added to the suspending agent, and the mixture is dried; a product is added to an internal mixer, the softener and the anti-ageing agent are added, and the mixture is mixed; secondary mixing is performed, and super glue is cooled; the super glue is added to sulfur and the vulcanization accelerator, and the rubber composition is obtained after being turned and batched out. Due to the special structure of the carbon nanotubes and the function of a group of the silane coupling agent, additives in the rubber are more uniformly mixed, the property of finished products of the rubber is more stable, and besides, the performance of the rubber in the aspects of mechanical abrasion resistance, corrosion resistance, rebound resilience, low-compression permanent deformation and the like is remarkably improved.