The invention discloses a mud and salt separation zero-discharge process of desulfurization wastewater. The process successively comprises a pretreatment process, a membrane treatment process and an evaporation crystallization process; in the pretreatment process, desulfurization wastewater enters into a raw water tank, an aeration fan introduces compressed air to the raw water tank, and then desulfurization wastewater is lifted to first-stage reaction and clarification through a raw water pump, and enters into a clear water tank after being subjected to three-stage softening and clarification, and one part of the clear water tank is provided for a membrane treatment process system, and the other part of the clear water tank is provided for a dosing system; incoming water in the membrane treatment process firstly is subjected to ultrafiltration and enters into a pH(Potential of Hydrogen) regulating box, and then is pumped to an nanofiltration membrane separation system and a reverse osmosis membrane separation system; incoming water in the evaporation crystallization process is firstly subjected to two-stage preheating and then enters into a degasser, finally enters into an evaporation concentration system and an evaporation crystallization system. Produced water of the process disclosed by the invention can be used as industrial water or boiler feedwater, by-products are maximally recycled to reduce the discharge of solid waste, and mud and salt are separated to reduce the disposal cost of the solid waste, and the by-products are recycled to produce certain economic benefits.